Some histochemical features of proteins of deciduocytes of the basal plate of the placenta in chronic basal deciduitis against the background of iron-deficiency anaemia in pregnant women
DOI:
https://doi.org/10.15574/PP.2025.2(102).1925Keywords:
placenta, chronic basal deciduitis, free radical processes, nitroperoxides, limited proteolysis, oxidative modification of proteins, iron-deficiency anemia of pregnant women, chemiluminescence, histochemistryAbstract
Free radicals and their impact on the placenta are the subject of active scientific research, as they may have significant implications for the health of pregnant women and fetal development.
Aim: to use histochemical methods to establish the quantitative characteristics of oxidative protein modification and limited proteolysis in deciduocytes of the basal plate of the placenta in cases of chronic basal deciduitis against the background of iron-deficiency anaemia in pregnant women.
Material and methods. A total of 82 placentas were examined using chemiluminescence and histochemical techniques, including methods by A. Yasuma and T. Ichikava, as well as bromophenol blue staining by Mikel Calvo and Bonheg.
Results. In cases of chronic basal deciduitis, the luminescence intensity of nitroperoxides in deciduocytes increased to 170±4.8 arbitrary units (arb. units). The quantitative analysis revealed an R/B (Red/Blue) coefficient, indicating the ratio of amino to carboxyl groups, of 2.34±0.01, and the optical density of histochemical staining for free amino groups of proteins was measured at 0.197±0.002 relative optical density units (rel. OD). These findings were statistically significant (p<0.001) when compared to placentas with inflammation but without anemia.
Conclusions. The activation of free radical processes appears to be the key factor driving the morphological characteristics of chronic basal deciduitis in iron-deficiency anemia of pregnant women. This is marked by an elevated concentration of peroxynitrites, resulting in enhanced oxidative protein modification and increased activity of limited proteolysis.
The study was conducted in accordance with the principles of the Declaration of Helsinki. The research protocol was approved by the Local Ethics Committee of the respective institution.
The author declares no conflict of interest.
References
Bagriy MM, Dibrova VA (editors). (2016). Methods of morphological research. Monograph. Vinnytsia: Nova Knyha: 328.
Baker BC, Heazell AE, Sibley C, Wright R, Bischof H, Beards F et al. (2021). Hypoxia and oxidative stress induce sterile placental inflammation in vitro. 11(1): 7281. https://doi.org/10.1038/s41598-021-86268-1; PMid:33790316 PMCid:PMC8012380
Ferreira T, Rasband W. (2019). ImageJ. User Guide. New York: National Institute of Health: 170. URL: https://imagej.nih.gov/ij/docs/guide/146.html.
Garvasiuk ОV. (2020). Pathological anatomy of premature maturation of the placenta with iron deficiency anemia in pregnancy. Dysertatsiya. Lviv: 281.
Goshovska AV, Hoshovsky VM, Harvasiuk OV. (2013). Chemiluminescent determination of nitroperoxides in the structures of placental chorionic villi. Praha: Education and Science s.r.o.: 3-6.
Guerby P, Tasta O, Swiader A, Pont F, Bujold E, Parant O, Vayssiere C, Salvayre R, Negre-Salvayre A. (2021). Role of oxidative stress in the dysfunction of the placental endothelial nitric oxide synthase in preeclampsia. Redox Biol. 40: 101861. https://doi.org/10.1016/j.redox.2021.101861; PMid:33548859 PMCid:PMC7873691
Hammer Ø. (2024). PAST: Paleontological Statistics. Version 4.16. Reference Manual. Oslo: Natural History Museum University of Oslo: 311.
Ilika VV, Garvasiuk OV, Dogolich OІ, Iryna BV. (2023). The features of limited proteolysis in placental fibrinoid in combination with inflammation and iron deficiency anemia of pregnant women. Wiad Lek. 76; 5 pt 1: 1022-1028. https://doi.org/10.36740/WLek202305121; PMid:37326085
Kasture V, Sundrani D, Randhir K, Wagh G, Joshi S. (2021). Placental apoptotic markers are associated with placental morphometry. Placenta. 115: 1-11. https://doi.org/10.1016/j.placenta.2021.08.051; PMid:34534910
Lien YC, Zhang Z, Barila G, Green-Brown A, Elovitz MA, Simmons RA. (2020). Intrauterine Inflammation Alters the Transcriptome and Metabolome in Placenta. Front Physiol. 11: 592689. https://doi.org/10.3389/fphys.2020.592689; PMid:33250783 PMCid:PMC7674943
Ohnieva VA. (2023). Social medicine, public health. Biological statistics. Kharkiv: KhNMU: 316.
Ruano CSM, Miralles F, Méhats C, Vaiman D. (2022). The Impact of Oxidative Stress of Environmental Origin on the Onset of Placental Diseases. Antioxidants. 11(1): 106. https://doi.org/10.3390/antiox11010106; PMid:35052610 PMCid:PMC8773163
Saroyo YB, Wibowo N, Irwinda R et al. (2021). Oxidative stress induced damage and early senescence in preterm placenta. J Pregnancy. 2021: 9923761. URL: https://www.hindawi.com/journals/jp/2021/9923761. https://doi.org/10.1155/2021/9923761; PMid:34258068 PMCid:PMC8249137
Shenderiuk OP, Davydenko IS. (2008). Oxidative modification of proteins in the syncytiotrophoblast cytoplasm of chorionic villi of the placenta in purulent chorionamnionitis (histochemical data)]. Svit medytsyny ta biolohii. 2(3): 88-90.
Soomro S. (2019). Oxidative stress and inflammation. Open Journal of Immunology. 9(1): 92086. https://doi.org/10.4236/oji.2019.91001
Sultana Z, Qiao Y, Maiti K, Smith R. (2023). Involvement of oxidative stress in placental dysfunction, the pathophysiology of fetal death and pregnancy disorders. Reproduction. 166(2): 25-38. https://doi.org/10.1530/REP-22-0278; PMid:37318094
Thomas MM, Haghiac M, Grozav C, Minium J, Calabuig-Navarro V, O'Tierney-Ginn P. (2019). Oxidative Stress Impairs Fatty Acid Oxidation and Mitochondrial Function in the Term Placenta. Reproductive Sciences. 26(7): 972-978. https://doi.org/10.1177/1933719118802054; PMid:30304995 PMCid:PMC6854426
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