Study of beta-glucuronidase activity as a marker of effectiveness of complex correction of symptomatic adenomyosis
DOI:
https://doi.org/10.15574/PP.2024.98.31Keywords:
symptomatic endometriosis, dienogest, elimination diet, beta-glucuronidase, womenAbstract
Disruption of the intestinal microflora and, in particular, an increase in the activity of bacterial β-glucuronidase, which is involved in estrogen metabolism and immune inflammation, is one of the probable factors in the development of adenomyosis.
Aim - to investigate the activity of fecal β-glucuronidase in patients with symptomatic adenomyosis and determine its significance as a laboratory marker of the effectiveness of complex correction of this pathology
Materials and methods. 150 women were examined - 120 patients with symptomatic adenomyosis who received standard treatment or an elimination diet depending on the detected type of food intolerance (study groups), and 30 healthy individuals without clinical and laboratory signs of inflammatory processes in the body and intestinal diseases (comparison group). The activity of β-glucuronidase in feces was determined for all of them
using phenolphthalein glucuronide as a substrate. Statistical data processing was carried out using the SPSS 21 program.
Results. Our results indicate that the activity of fecal β-glucuronidase in patients of the study groups was 1.5 times higher than in healthy women of the comparison group. Minimizing the consumption of disruptor products in combination with nutritional correction made it possible to reduce the frequency of complaints from the gastrointestinal tract. A significant decrease in β-glucuronidase activity was established in the study groups.
Conclusions. Determination of fecal β-glucuronidase activity can probably be an objective marker of the effectiveness of complex correction of symptomatic adenomyosis, which requires further research.
The research was carried out in accordance with the principles of the Helsinki Declaration. The study protocol was approved by the Local Ethics Committee of participating institution. The informed consent of the patient was obtained for conducting the studies.
No conflict of interest was declared by the authors.
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