Phenotypic features of immunocompetent cells in I-II pregnancy trimesters in women with anamnese infertility




CD-phenotype, immunocompetent cells, pregnant women, history of infertility, flow cytometry


It is impossible to solve the socio-economic problem of increasing the birth rate in Ukraine without determining the mechanisms of pregnancy disorders, among which immune ones occupy an important place, and developing individualized scientifically based therapy schemes.

Purpose - to determine the characteristics of levels of immunocompetent blood cells based on the CD phenotype in women with a history of infertility at different stages of pregnancy.

Materials and methods. With a FACSCan cell cytofloorimeter (Becton Dickinson, USA) and a test of Becton Dickinson systems (USA) determined using monoclonal antibodies (MCAs) to differentiated lymphocyte antigens, and activation markers (HLA-DR, CD25, CD69) or having inhibitory properties (CD158a) relative levels of immunocompetent cells 436 non-pregnant (reference group n) and 514 pregnant women with a history of infertility at different dates of the I and II trimesters of pregnancy (groups a-e).

Results. Against the background of reduced lymphocyte count and increased blood granulocytes in pregnant women, a significantly high relative level of T-L and T-cytotoxic subpopulations were found with their activation (CD3+CD8+HLA-DR+- and CD3+CD8+Cd In this gestation period there is also an increase as the number of T-helpers (CD3+CD4+-L), and their activation with expression on the membranes of HLA-DR- and CD25-molecules.

In the first 10 weeks of pregnancy, a decrease in the expression of inhibitory molecules CD158a (KIR2DL1) on NK T CD3+CD4+CD56+-cl and the relative level of B-lymphocytes (CD19+), as well as their subpopulations B-1a (CD19+CD5+) from 8 to 28 weeks was noted.

Conclusions. The peculiarities of the levels of peripheral blood immune system cells according to their CD phenotypes make it possible to observe the dynamics of the most important of them (CD3+CD8+HLA-DR+-, CD3+CD8+CD56+-, CD.

The research was carried out in accordance with the principles of the Helsinki declaration. The study protocol was approved by the Local ethics committee of the participating institution. The informed consent of the patient was obtained for conducting the studies.

No conflict of interests was declared by the authors.


Chernyshov VP, Sudoma IA, Donskoy BV, Goncharova YA. (2013). Multiple immune accentuations in women with ivf failures and efficacy of intravenous immunoglobulin administration. J. NAMN of Ukraine. 19: 212-218.

Donskoy BV, Chernyshov VP, Osypchuk DV. (2011). The imunophenotypic characteristics of two functionally different NK cells subpopulations in peripheral human blood . Physiol Journal: 29-35.

, Khaydukov SV, Zurochka AV. (2011). Analysis of T helper subpopulations (Th1, Th2, Treg, Th17, activated T-helpers) by means of flow cytometry. Medical Immunology. 13 (1): 7-16.

Kumar V, Medhi B. (2008). Emerging role of uterine natural killer cells in establishing pregnancy. Iran J Immunol. 5 (2): 71-81. URL:

Kushch OG, Zlobina OV. (2012). Quantitative and qualitative analysis of the distribution of CD5+-lymphocytes in the decidual cover of the uterus in I AND II periods of pregnancy in phystosis physis Current issues of modern medicine. 12, 3 (39): 147-150. URL:

Litvinova LS, Gutsol AA, Sokhonevich NA et al. (2014). Basic surface markers of functional activity T-lymphocytes. Medical Immunology. 16 (1): 7-26.

Morozov SA et al. (2020). Balance of CD4+IFNγγ+ and CD4+CD25hiT-cells as early predictor of a 3-month outcome in ischemic stroke patients. Medical Immunology. 22 (4): 675-684.

Smirnova TL, Portnova EV, Sergeeva VE. (2015). Immunity and pregnancy.

Warren HS, Campbell AJ, Waldron JC, Lanier LL. (2001). Biphasic response expressing both activating and inhibitory killer Ig-like. Intern Immunol. 13 (8): 1043-1052. URL:; PMid:11470774

Zurochka AV, Khaydukov SV, Kudryavtsev IV, Chereshnev VA. (2013). Protochnaya tsitometriya v meditsine i biologii. Yekaterinburg : RIO UrORAN: 552. Bibliogr: 483-551. ISBN 978-5-7691-2374-0.